Vicilin and Napin Storage-Protein Gene Promoters Are Responsive to Abscisic Acid in Developing Transgenic Tobacco Seed but Lose Sensitivity following Premature Desiccation
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چکیده
منابع مشابه
DREB2C interacts with ABF2, a bZIP protein regulating abscisic acid-responsive gene expression, and its overexpression affects abscisic acid sensitivity.
ABF2 is a basic leucine zipper protein that regulates abscisic acid (ABA)-dependent stress-responsive gene expression. We carried out yeast two-hybrid screens to isolate genes encoding ABF2-interacting proteins in Arabidopsis (Arabidopsis thaliana). Analysis of the resulting positive clones revealed that two of them encode an AP2 domain protein, which is the same as AtERF48/DREB2C. This protein...
متن کاملDistribution of napin and cruciferin in developing rape seed embryos.
The distribution of napin and cruciferin, the two major storage proteins in rape seed, Brassica napus, has been visualized during seed development by antibody staining of paraffin-embedded and sectioned seeds. The results indicate that the synthesis of both proteins during embryogenesis is strictly regulated with respect to time and tissue. Although the synthesis of napin started a few days ear...
متن کاملSeed Storage Protein Transcription and mRNA Levels in Brassica napus during Development and in Response to Exogenous Abscisic Acid.
Transcription rates and mRNA levels for Brassica napus seed storage protein families, cruciferin and napin, have been determined in embryos developing in the seed, as well as in embryos cultured with and without abscisic acid. Cruciferin and napin mRNAs are high during the cell expansion phase of embryo development, representing as much as 11 and 8%, respectively, of the total embryo mRNA. Duri...
متن کاملAna o 1, a cashew (Anacardium occidental) allergen of the vicilin seed storage protein family.
BACKGROUND The allergens responsible for cashew food allergy have not been well characterized. OBJECTIVES We initiated a study to clone cDNAs encoding cashew food allergens. METHODS A cashew cDNA library was screened with human serum for IgE-reactive clones and rabbit IgG anti-cashew extract antisera. Reactive clones were sequenced and expressed, and linear epitopes were identified by means...
متن کاملRole of Abscisic Acid in the Induction of Desiccation Tolerance in Developing Seeds of Arabidopsis thaliana.
In contrast to wild-type seeds of Arabidopsis thaliana and to seeds deficient in (aba) or insensitive to (abi3) abscisic acid (ABA), maturing seeds of recombinant (aba,abi3) plants fail to desiccate, remain green, and lose viability upon drying. These double-mutant seeds acquire only low levels of the major storage proteins and are deficient in several low mol wt polypeptides, both soluble and ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1996
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.110.4.1135